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    Bio-Rad bio rad itaq universal sybr green supermix
    Bio Rad Itaq Universal Sybr Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 8833 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ssoadvanced+universal+sybr+green+supermix+bio+rad/SsoAdvanced+Universal+SYBR+Green+Supermix/pm42032219-311-5-5
    Average 99 stars, based on 8833 article reviews
    bio rad itaq universal sybr green supermix - by Bioz Stars, 2026-09
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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: ADAR1 Controls Macrophage Scavenging and Lipid‐Buffering Programs in Metabolic Tissues
    Article Snippet: Reverse transcription was performed with the iScript reverse transcription reagent kit (Bio‐Rad) according to the manufacturer's protocol. .. Quantitative PCR (qPCR) for ADAR and TBP expression was performed using the SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad) on the CFX96 Real‐Time PCR System (Bio‐Rad). ..

    Article Title: Telomerase Depletion Accelerates Ageing of the Zebrafish Brain
    Article Snippet: .. cDNA samples from zebrafish brains were analysed by real‐time qPCR using SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad), forward and reverse primers at a final concentration of 200 nM (Table ), and cDNA diluted 1:40. ..

    Article Title: Metabolic Profiling Reveals a Glycolytic Shift and an IRG1 /Itaconate/ NF2L2 Axis Regulating Neurotoxic Oxidative Stress in Inflammatory Microglia
    Article Snippet: The cDNA was synthesized from 1 μg of RNA using Oligo dT, dNTPs, and Superscript IV (Invitrogen) with a Bio‐Rad T100 PCR Thermal Cycler. .. RT‐qPCR was performed using SSoAdvanced Universal SYBR Green Supermix (Bio‐Rad) and run on a CFX Duet Real‐Time PCR System (Bio‐Rad). ..

    Article Title: Boron Nitride Nanomaterials Trigger Immunomodulatory Effects in Human Broncho‐Epithelial Cells by Modulating Eicosanoid Lipid Signaling
    Article Snippet: .. [ ] For qPCR reaction (Biorad), 50 ng of cDNA was added in predesigned 96‐well PrimePCR plates (Bio‐Rad) followed by adding SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad) for a 20 μL reaction. ..

    Article Title: Fasting and Caloric Restriction Activate an ADIOL ‐ NHR ‐91‐Kynurenine Pathway Signaling Axis to Promote Healthspan
    Article Snippet: First‐strand cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) with random primers. .. Quantitative PCR was performed using the SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad) on a C100 Touch Thermal Cycler CFX384 Real‐Time System (Bio‐Rad), in 384‐well plates. ..

    Expressing:

    Article Title: ADAR1 Controls Macrophage Scavenging and Lipid‐Buffering Programs in Metabolic Tissues
    Article Snippet: Reverse transcription was performed with the iScript reverse transcription reagent kit (Bio‐Rad) according to the manufacturer's protocol. .. Quantitative PCR (qPCR) for ADAR and TBP expression was performed using the SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad) on the CFX96 Real‐Time PCR System (Bio‐Rad). ..

    SYBR Green Assay:

    Article Title: ADAR1 Controls Macrophage Scavenging and Lipid‐Buffering Programs in Metabolic Tissues
    Article Snippet: Reverse transcription was performed with the iScript reverse transcription reagent kit (Bio‐Rad) according to the manufacturer's protocol. .. Quantitative PCR (qPCR) for ADAR and TBP expression was performed using the SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad) on the CFX96 Real‐Time PCR System (Bio‐Rad). ..

    Article Title: Telomerase Depletion Accelerates Ageing of the Zebrafish Brain
    Article Snippet: .. cDNA samples from zebrafish brains were analysed by real‐time qPCR using SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad), forward and reverse primers at a final concentration of 200 nM (Table ), and cDNA diluted 1:40. ..

    Article Title: Metabolic Profiling Reveals a Glycolytic Shift and an IRG1 /Itaconate/ NF2L2 Axis Regulating Neurotoxic Oxidative Stress in Inflammatory Microglia
    Article Snippet: The cDNA was synthesized from 1 μg of RNA using Oligo dT, dNTPs, and Superscript IV (Invitrogen) with a Bio‐Rad T100 PCR Thermal Cycler. .. RT‐qPCR was performed using SSoAdvanced Universal SYBR Green Supermix (Bio‐Rad) and run on a CFX Duet Real‐Time PCR System (Bio‐Rad). ..

    Article Title: Boron Nitride Nanomaterials Trigger Immunomodulatory Effects in Human Broncho‐Epithelial Cells by Modulating Eicosanoid Lipid Signaling
    Article Snippet: .. [ ] For qPCR reaction (Biorad), 50 ng of cDNA was added in predesigned 96‐well PrimePCR plates (Bio‐Rad) followed by adding SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad) for a 20 μL reaction. ..

    Article Title: Identification of novel type 1 and type 2 diabetes genes by co-localization of human islet eQTL and GWAS variants with colocRedRibbon.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Prototype strain of enterovirus, CVB1/Conn-5 ATCC ATCC VR-28 Chemicals, peptides, and recombinant proteins Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat #13778150 Propidium iodide Sigma-Aldrich Cat#P4170; CAS: 25535-16-4 Hoechst 33342 (bisBenzimide H 33342 trihydrochloride) Sigma-Aldrich Cat#14533; CAS: 875756-97-1 Forskolin Sigma-Aldrich Cat#F6886; CAS: 66575-29-9 Beta Krebs Human Cell Design Group Cat#BK-100 SsoAdvanced Universal SYBR Green Supermix Bio-Rad CAT1725274 Critical commercial assays Human Insulin ELISA Mercodia Cat #10-1113-10 Invitrogen TM PureLink TM RNA Micro Scale Kit Invitrogen Cat#12183016 Applied Biosystems TM TaqMan TM Reverse Transcription Reagents Applied Biosystems Cat#4304134 Deposited data eQTL Alonso et al. 17 http://tiger.bsc.es/ pQTL Ferkingstad et al. 21 N/A meQTL McAllan et al. 22 N/A GWAS Chen et al., 11 Suzuki et al., 15 Kurki et al., 23 Yengo et al. 24 N/A Co-localization list, plots and source code This paper https://doi.org/10.5281/zenodo.13987433 Experimental models: Cell lines EndoC-βH1 Ravassard et al. 66 RRID:CVCL_L909 Oligonucleotides AllStars Negative Control siRNA Qiagen Cat #1027281 Human siFUT2, Stealth siRNA Thermofisher Scientific ID:HSS103862 Human SiFUT2#2, Silencer Select siRNA Thermofisher Scientific ID:S533826 Primer for FUT2 Forward: AGCGGCTAGCGAA GATTCA This paper N/A Primer for FUT2 Reverse: GGTCCCAGTGCCT TTGATGT This paper N/A Primer for ACTB Forward: CTGTACGCCAACA CAGTGCT Costa-Junior et al. 67 N/A Primer for ACTB Reverse: GCTCAGGAGGAGC AATGATC Costa-Junior et al. 67 N/A Primer for VAPA Forward: TACCGAAACAAGGA AACTAATGGAA Costa-Junior et al. 67 N/A Primer for VAPA Reverse: GCCTTAAACCTTCA TCTCTCAGGT Costa-Junior et al. 67 N/A Software and algorithms colocRedRibbon This paper https://github.com/antpiron/colocRedRibbon and https://doi.org/10.5281/zenodo.13987433 (version used for this paper) RedRibbon Piron et al. 26 https://github.com/antpiron/RedRibbon (Continued on next page) e1 Cell Genomics 5, 101004, November 12, 2025 OPEN ACCESS ..

    Article Title: Subgenomic Distribution and Herbicide Cross‐Resistance of ALS Gene Mutations in Allohexaploid Echinochloa crus‐galli
    Article Snippet: .. The master mix used was SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad) following the manufacturer's protocol instructions, 98°C for 3 min and 40 cycles of 98°C for 15 s and “annealing temperature” for 30s, followed by one step of 65°C for 5 s and 95°C for 50s, utilizing the equipment CFX96 Real‐time System (Bio‐Rad). ..

    Article Title: Lung adenocarcinoma surfaceome remodeling with EGFR inhibitors uncovers placental alkaline phosphatase as a target for combination therapy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALPP antibody (8B6) Novus Cat# NB110-3638; RRID:AB_787958) ALPP antibody (EPR6141) Abcam Cat# ab133602; RRID:AB_3065177) β-Actin antibody (8H10D10) Cell Signaling Technology Cat# 3700; RRID:AB_2242334 Phospho-Akt (Ser473) Antibody Cell Signaling Technology Cat# 9271; RRID:AB_329825 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody Cell Signaling Technology Cat# 9101; RRID:AB_331646 Phospho-Stat1 (Tyr701) (58D6) Antibody Cell Signaling Technology Cat# 9167; RRID:AB_561284 Phospho-Stat1 (Ser727) (D3B7) Antibody Cell Signaling Technology Cat# 8826; RRID:AB_2773718 Akt (pan) (C67E7) Antibody Cell Signaling Technology Cat# 4691; RRID:AB_915783 p44/42 MAPK (Erk1/2) (137F5) Antibody Cell Signaling Technology Cat# 4695; RRID:AB_390779 Stat1 (D1K9Y) Antibody Cell Signaling Technology Cat# 14994; RRID:AB_2737027 GAPDH (D16H11) XP Antibody Cell Signaling Technology Cat# 5174; RRID:AB_10622025 FoxO3a (D19A7) Antibody Cell Signaling Technology Cat# 12829; RRID:AB_2636990 Phospho-FoxO3a (Ser294) Antibody Cell Signaling Technology Cat# 5538; RRID:AB_10696878 Phospho-FoxO3a (Ser425) (D5E9T) Antibody Cell Signaling Technology Cat# 64616; RRID:AB_2799662 Biological samples Human Multi-tissue Tissue Microarray (Normal) Novus Biologicals Cat# NBP2-78113 TMA This manuscript The University of Texas MD Anderson Cancer Center Chemicals, peptides, and recombinant proteins FoxO inhibitor MedChemExpress Cat# AS1842856 CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega Cat# G3581 D-Luciferin potassium salt Regis Technologies Inc Cat# 115144-35-9 Gefitinib Cayman Chemical Company Cat# 13166 Lapatinib Cayman Chemical Company Cat# 11493-5 Afatibib Selleck Chemicals Cat# S1011 Osimertinib Selleck Chemicals Cat# S7297 EGFR inhibitor Cayman Chemical Company Cat# 15363 EGFR/ERBB2 inhibitor Cayman Chemical Company Cat# 17568 SsoAdvanced Universal SYBR Green Supermix BIO-RAD Cat# 1725271 16% Formaldehyde, Methanol-free Thermo Fisher Scientific Cat# 28906 DMSO Sigma-Aldrich Cat# D2650 TaqMan Universal Master Mix II, no UNG Applied Biosystems Cat# 4440040 NVP-BKM120 Cayman Chemical Company Cat# 11587 AZD8330 MedChemExpress Cat# HY-12058 SCH772984 MedChemExpress Cat# HY-50846 Critical commercial assays H&E staining kit Abcam Cat# Ab245880 Cell Fractionation kit Cell Signaling Technology Cat# 9038 Pierce Magnetic ChIP kit Thermo Fisher Scientific Cat# 26157 Deposited data PRIDE database This paper PXD069074 (Continued on next page) Cell Reports Medicine 6, 102513, December 16, 2025 e1 ..

    Article Title: Fasting and Caloric Restriction Activate an ADIOL ‐ NHR ‐91‐Kynurenine Pathway Signaling Axis to Promote Healthspan
    Article Snippet: First‐strand cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) with random primers. .. Quantitative PCR was performed using the SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad) on a C100 Touch Thermal Cycler CFX384 Real‐Time System (Bio‐Rad), in 384‐well plates. ..

    Concentration Assay:

    Article Title: Telomerase Depletion Accelerates Ageing of the Zebrafish Brain
    Article Snippet: .. cDNA samples from zebrafish brains were analysed by real‐time qPCR using SsoAdvanced Universal SYBR Green Supermix (Bio‐Rad), forward and reverse primers at a final concentration of 200 nM (Table ), and cDNA diluted 1:40. ..

    Virus:

    Article Title: Identification of novel type 1 and type 2 diabetes genes by co-localization of human islet eQTL and GWAS variants with colocRedRibbon.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Prototype strain of enterovirus, CVB1/Conn-5 ATCC ATCC VR-28 Chemicals, peptides, and recombinant proteins Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat #13778150 Propidium iodide Sigma-Aldrich Cat#P4170; CAS: 25535-16-4 Hoechst 33342 (bisBenzimide H 33342 trihydrochloride) Sigma-Aldrich Cat#14533; CAS: 875756-97-1 Forskolin Sigma-Aldrich Cat#F6886; CAS: 66575-29-9 Beta Krebs Human Cell Design Group Cat#BK-100 SsoAdvanced Universal SYBR Green Supermix Bio-Rad CAT1725274 Critical commercial assays Human Insulin ELISA Mercodia Cat #10-1113-10 Invitrogen TM PureLink TM RNA Micro Scale Kit Invitrogen Cat#12183016 Applied Biosystems TM TaqMan TM Reverse Transcription Reagents Applied Biosystems Cat#4304134 Deposited data eQTL Alonso et al. 17 http://tiger.bsc.es/ pQTL Ferkingstad et al. 21 N/A meQTL McAllan et al. 22 N/A GWAS Chen et al., 11 Suzuki et al., 15 Kurki et al., 23 Yengo et al. 24 N/A Co-localization list, plots and source code This paper https://doi.org/10.5281/zenodo.13987433 Experimental models: Cell lines EndoC-βH1 Ravassard et al. 66 RRID:CVCL_L909 Oligonucleotides AllStars Negative Control siRNA Qiagen Cat #1027281 Human siFUT2, Stealth siRNA Thermofisher Scientific ID:HSS103862 Human SiFUT2#2, Silencer Select siRNA Thermofisher Scientific ID:S533826 Primer for FUT2 Forward: AGCGGCTAGCGAA GATTCA This paper N/A Primer for FUT2 Reverse: GGTCCCAGTGCCT TTGATGT This paper N/A Primer for ACTB Forward: CTGTACGCCAACA CAGTGCT Costa-Junior et al. 67 N/A Primer for ACTB Reverse: GCTCAGGAGGAGC AATGATC Costa-Junior et al. 67 N/A Primer for VAPA Forward: TACCGAAACAAGGA AACTAATGGAA Costa-Junior et al. 67 N/A Primer for VAPA Reverse: GCCTTAAACCTTCA TCTCTCAGGT Costa-Junior et al. 67 N/A Software and algorithms colocRedRibbon This paper https://github.com/antpiron/colocRedRibbon and https://doi.org/10.5281/zenodo.13987433 (version used for this paper) RedRibbon Piron et al. 26 https://github.com/antpiron/RedRibbon (Continued on next page) e1 Cell Genomics 5, 101004, November 12, 2025 OPEN ACCESS ..

    Recombinant:

    Article Title: Identification of novel type 1 and type 2 diabetes genes by co-localization of human islet eQTL and GWAS variants with colocRedRibbon.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Prototype strain of enterovirus, CVB1/Conn-5 ATCC ATCC VR-28 Chemicals, peptides, and recombinant proteins Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat #13778150 Propidium iodide Sigma-Aldrich Cat#P4170; CAS: 25535-16-4 Hoechst 33342 (bisBenzimide H 33342 trihydrochloride) Sigma-Aldrich Cat#14533; CAS: 875756-97-1 Forskolin Sigma-Aldrich Cat#F6886; CAS: 66575-29-9 Beta Krebs Human Cell Design Group Cat#BK-100 SsoAdvanced Universal SYBR Green Supermix Bio-Rad CAT1725274 Critical commercial assays Human Insulin ELISA Mercodia Cat #10-1113-10 Invitrogen TM PureLink TM RNA Micro Scale Kit Invitrogen Cat#12183016 Applied Biosystems TM TaqMan TM Reverse Transcription Reagents Applied Biosystems Cat#4304134 Deposited data eQTL Alonso et al. 17 http://tiger.bsc.es/ pQTL Ferkingstad et al. 21 N/A meQTL McAllan et al. 22 N/A GWAS Chen et al., 11 Suzuki et al., 15 Kurki et al., 23 Yengo et al. 24 N/A Co-localization list, plots and source code This paper https://doi.org/10.5281/zenodo.13987433 Experimental models: Cell lines EndoC-βH1 Ravassard et al. 66 RRID:CVCL_L909 Oligonucleotides AllStars Negative Control siRNA Qiagen Cat #1027281 Human siFUT2, Stealth siRNA Thermofisher Scientific ID:HSS103862 Human SiFUT2#2, Silencer Select siRNA Thermofisher Scientific ID:S533826 Primer for FUT2 Forward: AGCGGCTAGCGAA GATTCA This paper N/A Primer for FUT2 Reverse: GGTCCCAGTGCCT TTGATGT This paper N/A Primer for ACTB Forward: CTGTACGCCAACA CAGTGCT Costa-Junior et al. 67 N/A Primer for ACTB Reverse: GCTCAGGAGGAGC AATGATC Costa-Junior et al. 67 N/A Primer for VAPA Forward: TACCGAAACAAGGA AACTAATGGAA Costa-Junior et al. 67 N/A Primer for VAPA Reverse: GCCTTAAACCTTCA TCTCTCAGGT Costa-Junior et al. 67 N/A Software and algorithms colocRedRibbon This paper https://github.com/antpiron/colocRedRibbon and https://doi.org/10.5281/zenodo.13987433 (version used for this paper) RedRibbon Piron et al. 26 https://github.com/antpiron/RedRibbon (Continued on next page) e1 Cell Genomics 5, 101004, November 12, 2025 OPEN ACCESS ..

    Article Title: Lung adenocarcinoma surfaceome remodeling with EGFR inhibitors uncovers placental alkaline phosphatase as a target for combination therapy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALPP antibody (8B6) Novus Cat# NB110-3638; RRID:AB_787958) ALPP antibody (EPR6141) Abcam Cat# ab133602; RRID:AB_3065177) β-Actin antibody (8H10D10) Cell Signaling Technology Cat# 3700; RRID:AB_2242334 Phospho-Akt (Ser473) Antibody Cell Signaling Technology Cat# 9271; RRID:AB_329825 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody Cell Signaling Technology Cat# 9101; RRID:AB_331646 Phospho-Stat1 (Tyr701) (58D6) Antibody Cell Signaling Technology Cat# 9167; RRID:AB_561284 Phospho-Stat1 (Ser727) (D3B7) Antibody Cell Signaling Technology Cat# 8826; RRID:AB_2773718 Akt (pan) (C67E7) Antibody Cell Signaling Technology Cat# 4691; RRID:AB_915783 p44/42 MAPK (Erk1/2) (137F5) Antibody Cell Signaling Technology Cat# 4695; RRID:AB_390779 Stat1 (D1K9Y) Antibody Cell Signaling Technology Cat# 14994; RRID:AB_2737027 GAPDH (D16H11) XP Antibody Cell Signaling Technology Cat# 5174; RRID:AB_10622025 FoxO3a (D19A7) Antibody Cell Signaling Technology Cat# 12829; RRID:AB_2636990 Phospho-FoxO3a (Ser294) Antibody Cell Signaling Technology Cat# 5538; RRID:AB_10696878 Phospho-FoxO3a (Ser425) (D5E9T) Antibody Cell Signaling Technology Cat# 64616; RRID:AB_2799662 Biological samples Human Multi-tissue Tissue Microarray (Normal) Novus Biologicals Cat# NBP2-78113 TMA This manuscript The University of Texas MD Anderson Cancer Center Chemicals, peptides, and recombinant proteins FoxO inhibitor MedChemExpress Cat# AS1842856 CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega Cat# G3581 D-Luciferin potassium salt Regis Technologies Inc Cat# 115144-35-9 Gefitinib Cayman Chemical Company Cat# 13166 Lapatinib Cayman Chemical Company Cat# 11493-5 Afatibib Selleck Chemicals Cat# S1011 Osimertinib Selleck Chemicals Cat# S7297 EGFR inhibitor Cayman Chemical Company Cat# 15363 EGFR/ERBB2 inhibitor Cayman Chemical Company Cat# 17568 SsoAdvanced Universal SYBR Green Supermix BIO-RAD Cat# 1725271 16% Formaldehyde, Methanol-free Thermo Fisher Scientific Cat# 28906 DMSO Sigma-Aldrich Cat# D2650 TaqMan Universal Master Mix II, no UNG Applied Biosystems Cat# 4440040 NVP-BKM120 Cayman Chemical Company Cat# 11587 AZD8330 MedChemExpress Cat# HY-12058 SCH772984 MedChemExpress Cat# HY-50846 Critical commercial assays H&E staining kit Abcam Cat# Ab245880 Cell Fractionation kit Cell Signaling Technology Cat# 9038 Pierce Magnetic ChIP kit Thermo Fisher Scientific Cat# 26157 Deposited data PRIDE database This paper PXD069074 (Continued on next page) Cell Reports Medicine 6, 102513, December 16, 2025 e1 ..

    Transfection:

    Article Title: Identification of novel type 1 and type 2 diabetes genes by co-localization of human islet eQTL and GWAS variants with colocRedRibbon.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Prototype strain of enterovirus, CVB1/Conn-5 ATCC ATCC VR-28 Chemicals, peptides, and recombinant proteins Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat #13778150 Propidium iodide Sigma-Aldrich Cat#P4170; CAS: 25535-16-4 Hoechst 33342 (bisBenzimide H 33342 trihydrochloride) Sigma-Aldrich Cat#14533; CAS: 875756-97-1 Forskolin Sigma-Aldrich Cat#F6886; CAS: 66575-29-9 Beta Krebs Human Cell Design Group Cat#BK-100 SsoAdvanced Universal SYBR Green Supermix Bio-Rad CAT1725274 Critical commercial assays Human Insulin ELISA Mercodia Cat #10-1113-10 Invitrogen TM PureLink TM RNA Micro Scale Kit Invitrogen Cat#12183016 Applied Biosystems TM TaqMan TM Reverse Transcription Reagents Applied Biosystems Cat#4304134 Deposited data eQTL Alonso et al. 17 http://tiger.bsc.es/ pQTL Ferkingstad et al. 21 N/A meQTL McAllan et al. 22 N/A GWAS Chen et al., 11 Suzuki et al., 15 Kurki et al., 23 Yengo et al. 24 N/A Co-localization list, plots and source code This paper https://doi.org/10.5281/zenodo.13987433 Experimental models: Cell lines EndoC-βH1 Ravassard et al. 66 RRID:CVCL_L909 Oligonucleotides AllStars Negative Control siRNA Qiagen Cat #1027281 Human siFUT2, Stealth siRNA Thermofisher Scientific ID:HSS103862 Human SiFUT2#2, Silencer Select siRNA Thermofisher Scientific ID:S533826 Primer for FUT2 Forward: AGCGGCTAGCGAA GATTCA This paper N/A Primer for FUT2 Reverse: GGTCCCAGTGCCT TTGATGT This paper N/A Primer for ACTB Forward: CTGTACGCCAACA CAGTGCT Costa-Junior et al. 67 N/A Primer for ACTB Reverse: GCTCAGGAGGAGC AATGATC Costa-Junior et al. 67 N/A Primer for VAPA Forward: TACCGAAACAAGGA AACTAATGGAA Costa-Junior et al. 67 N/A Primer for VAPA Reverse: GCCTTAAACCTTCA TCTCTCAGGT Costa-Junior et al. 67 N/A Software and algorithms colocRedRibbon This paper https://github.com/antpiron/colocRedRibbon and https://doi.org/10.5281/zenodo.13987433 (version used for this paper) RedRibbon Piron et al. 26 https://github.com/antpiron/RedRibbon (Continued on next page) e1 Cell Genomics 5, 101004, November 12, 2025 OPEN ACCESS ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Identification of novel type 1 and type 2 diabetes genes by co-localization of human islet eQTL and GWAS variants with colocRedRibbon.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Prototype strain of enterovirus, CVB1/Conn-5 ATCC ATCC VR-28 Chemicals, peptides, and recombinant proteins Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat #13778150 Propidium iodide Sigma-Aldrich Cat#P4170; CAS: 25535-16-4 Hoechst 33342 (bisBenzimide H 33342 trihydrochloride) Sigma-Aldrich Cat#14533; CAS: 875756-97-1 Forskolin Sigma-Aldrich Cat#F6886; CAS: 66575-29-9 Beta Krebs Human Cell Design Group Cat#BK-100 SsoAdvanced Universal SYBR Green Supermix Bio-Rad CAT1725274 Critical commercial assays Human Insulin ELISA Mercodia Cat #10-1113-10 Invitrogen TM PureLink TM RNA Micro Scale Kit Invitrogen Cat#12183016 Applied Biosystems TM TaqMan TM Reverse Transcription Reagents Applied Biosystems Cat#4304134 Deposited data eQTL Alonso et al. 17 http://tiger.bsc.es/ pQTL Ferkingstad et al. 21 N/A meQTL McAllan et al. 22 N/A GWAS Chen et al., 11 Suzuki et al., 15 Kurki et al., 23 Yengo et al. 24 N/A Co-localization list, plots and source code This paper https://doi.org/10.5281/zenodo.13987433 Experimental models: Cell lines EndoC-βH1 Ravassard et al. 66 RRID:CVCL_L909 Oligonucleotides AllStars Negative Control siRNA Qiagen Cat #1027281 Human siFUT2, Stealth siRNA Thermofisher Scientific ID:HSS103862 Human SiFUT2#2, Silencer Select siRNA Thermofisher Scientific ID:S533826 Primer for FUT2 Forward: AGCGGCTAGCGAA GATTCA This paper N/A Primer for FUT2 Reverse: GGTCCCAGTGCCT TTGATGT This paper N/A Primer for ACTB Forward: CTGTACGCCAACA CAGTGCT Costa-Junior et al. 67 N/A Primer for ACTB Reverse: GCTCAGGAGGAGC AATGATC Costa-Junior et al. 67 N/A Primer for VAPA Forward: TACCGAAACAAGGA AACTAATGGAA Costa-Junior et al. 67 N/A Primer for VAPA Reverse: GCCTTAAACCTTCA TCTCTCAGGT Costa-Junior et al. 67 N/A Software and algorithms colocRedRibbon This paper https://github.com/antpiron/colocRedRibbon and https://doi.org/10.5281/zenodo.13987433 (version used for this paper) RedRibbon Piron et al. 26 https://github.com/antpiron/RedRibbon (Continued on next page) e1 Cell Genomics 5, 101004, November 12, 2025 OPEN ACCESS ..

    Reverse Transcription:

    Article Title: Identification of novel type 1 and type 2 diabetes genes by co-localization of human islet eQTL and GWAS variants with colocRedRibbon.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Prototype strain of enterovirus, CVB1/Conn-5 ATCC ATCC VR-28 Chemicals, peptides, and recombinant proteins Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat #13778150 Propidium iodide Sigma-Aldrich Cat#P4170; CAS: 25535-16-4 Hoechst 33342 (bisBenzimide H 33342 trihydrochloride) Sigma-Aldrich Cat#14533; CAS: 875756-97-1 Forskolin Sigma-Aldrich Cat#F6886; CAS: 66575-29-9 Beta Krebs Human Cell Design Group Cat#BK-100 SsoAdvanced Universal SYBR Green Supermix Bio-Rad CAT1725274 Critical commercial assays Human Insulin ELISA Mercodia Cat #10-1113-10 Invitrogen TM PureLink TM RNA Micro Scale Kit Invitrogen Cat#12183016 Applied Biosystems TM TaqMan TM Reverse Transcription Reagents Applied Biosystems Cat#4304134 Deposited data eQTL Alonso et al. 17 http://tiger.bsc.es/ pQTL Ferkingstad et al. 21 N/A meQTL McAllan et al. 22 N/A GWAS Chen et al., 11 Suzuki et al., 15 Kurki et al., 23 Yengo et al. 24 N/A Co-localization list, plots and source code This paper https://doi.org/10.5281/zenodo.13987433 Experimental models: Cell lines EndoC-βH1 Ravassard et al. 66 RRID:CVCL_L909 Oligonucleotides AllStars Negative Control siRNA Qiagen Cat #1027281 Human siFUT2, Stealth siRNA Thermofisher Scientific ID:HSS103862 Human SiFUT2#2, Silencer Select siRNA Thermofisher Scientific ID:S533826 Primer for FUT2 Forward: AGCGGCTAGCGAA GATTCA This paper N/A Primer for FUT2 Reverse: GGTCCCAGTGCCT TTGATGT This paper N/A Primer for ACTB Forward: CTGTACGCCAACA CAGTGCT Costa-Junior et al. 67 N/A Primer for ACTB Reverse: GCTCAGGAGGAGC AATGATC Costa-Junior et al. 67 N/A Primer for VAPA Forward: TACCGAAACAAGGA AACTAATGGAA Costa-Junior et al. 67 N/A Primer for VAPA Reverse: GCCTTAAACCTTCA TCTCTCAGGT Costa-Junior et al. 67 N/A Software and algorithms colocRedRibbon This paper https://github.com/antpiron/colocRedRibbon and https://doi.org/10.5281/zenodo.13987433 (version used for this paper) RedRibbon Piron et al. 26 https://github.com/antpiron/RedRibbon (Continued on next page) e1 Cell Genomics 5, 101004, November 12, 2025 OPEN ACCESS ..

    GWAS:

    Article Title: Identification of novel type 1 and type 2 diabetes genes by co-localization of human islet eQTL and GWAS variants with colocRedRibbon.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Prototype strain of enterovirus, CVB1/Conn-5 ATCC ATCC VR-28 Chemicals, peptides, and recombinant proteins Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat #13778150 Propidium iodide Sigma-Aldrich Cat#P4170; CAS: 25535-16-4 Hoechst 33342 (bisBenzimide H 33342 trihydrochloride) Sigma-Aldrich Cat#14533; CAS: 875756-97-1 Forskolin Sigma-Aldrich Cat#F6886; CAS: 66575-29-9 Beta Krebs Human Cell Design Group Cat#BK-100 SsoAdvanced Universal SYBR Green Supermix Bio-Rad CAT1725274 Critical commercial assays Human Insulin ELISA Mercodia Cat #10-1113-10 Invitrogen TM PureLink TM RNA Micro Scale Kit Invitrogen Cat#12183016 Applied Biosystems TM TaqMan TM Reverse Transcription Reagents Applied Biosystems Cat#4304134 Deposited data eQTL Alonso et al. 17 http://tiger.bsc.es/ pQTL Ferkingstad et al. 21 N/A meQTL McAllan et al. 22 N/A GWAS Chen et al., 11 Suzuki et al., 15 Kurki et al., 23 Yengo et al. 24 N/A Co-localization list, plots and source code This paper https://doi.org/10.5281/zenodo.13987433 Experimental models: Cell lines EndoC-βH1 Ravassard et al. 66 RRID:CVCL_L909 Oligonucleotides AllStars Negative Control siRNA Qiagen Cat #1027281 Human siFUT2, Stealth siRNA Thermofisher Scientific ID:HSS103862 Human SiFUT2#2, Silencer Select siRNA Thermofisher Scientific ID:S533826 Primer for FUT2 Forward: AGCGGCTAGCGAA GATTCA This paper N/A Primer for FUT2 Reverse: GGTCCCAGTGCCT TTGATGT This paper N/A Primer for ACTB Forward: CTGTACGCCAACA CAGTGCT Costa-Junior et al. 67 N/A Primer for ACTB Reverse: GCTCAGGAGGAGC AATGATC Costa-Junior et al. 67 N/A Primer for VAPA Forward: TACCGAAACAAGGA AACTAATGGAA Costa-Junior et al. 67 N/A Primer for VAPA Reverse: GCCTTAAACCTTCA TCTCTCAGGT Costa-Junior et al. 67 N/A Software and algorithms colocRedRibbon This paper https://github.com/antpiron/colocRedRibbon and https://doi.org/10.5281/zenodo.13987433 (version used for this paper) RedRibbon Piron et al. 26 https://github.com/antpiron/RedRibbon (Continued on next page) e1 Cell Genomics 5, 101004, November 12, 2025 OPEN ACCESS ..

    Negative Control:

    Article Title: Identification of novel type 1 and type 2 diabetes genes by co-localization of human islet eQTL and GWAS variants with colocRedRibbon.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Prototype strain of enterovirus, CVB1/Conn-5 ATCC ATCC VR-28 Chemicals, peptides, and recombinant proteins Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat #13778150 Propidium iodide Sigma-Aldrich Cat#P4170; CAS: 25535-16-4 Hoechst 33342 (bisBenzimide H 33342 trihydrochloride) Sigma-Aldrich Cat#14533; CAS: 875756-97-1 Forskolin Sigma-Aldrich Cat#F6886; CAS: 66575-29-9 Beta Krebs Human Cell Design Group Cat#BK-100 SsoAdvanced Universal SYBR Green Supermix Bio-Rad CAT1725274 Critical commercial assays Human Insulin ELISA Mercodia Cat #10-1113-10 Invitrogen TM PureLink TM RNA Micro Scale Kit Invitrogen Cat#12183016 Applied Biosystems TM TaqMan TM Reverse Transcription Reagents Applied Biosystems Cat#4304134 Deposited data eQTL Alonso et al. 17 http://tiger.bsc.es/ pQTL Ferkingstad et al. 21 N/A meQTL McAllan et al. 22 N/A GWAS Chen et al., 11 Suzuki et al., 15 Kurki et al., 23 Yengo et al. 24 N/A Co-localization list, plots and source code This paper https://doi.org/10.5281/zenodo.13987433 Experimental models: Cell lines EndoC-βH1 Ravassard et al. 66 RRID:CVCL_L909 Oligonucleotides AllStars Negative Control siRNA Qiagen Cat #1027281 Human siFUT2, Stealth siRNA Thermofisher Scientific ID:HSS103862 Human SiFUT2#2, Silencer Select siRNA Thermofisher Scientific ID:S533826 Primer for FUT2 Forward: AGCGGCTAGCGAA GATTCA This paper N/A Primer for FUT2 Reverse: GGTCCCAGTGCCT TTGATGT This paper N/A Primer for ACTB Forward: CTGTACGCCAACA CAGTGCT Costa-Junior et al. 67 N/A Primer for ACTB Reverse: GCTCAGGAGGAGC AATGATC Costa-Junior et al. 67 N/A Primer for VAPA Forward: TACCGAAACAAGGA AACTAATGGAA Costa-Junior et al. 67 N/A Primer for VAPA Reverse: GCCTTAAACCTTCA TCTCTCAGGT Costa-Junior et al. 67 N/A Software and algorithms colocRedRibbon This paper https://github.com/antpiron/colocRedRibbon and https://doi.org/10.5281/zenodo.13987433 (version used for this paper) RedRibbon Piron et al. 26 https://github.com/antpiron/RedRibbon (Continued on next page) e1 Cell Genomics 5, 101004, November 12, 2025 OPEN ACCESS ..

    Software:

    Article Title: Identification of novel type 1 and type 2 diabetes genes by co-localization of human islet eQTL and GWAS variants with colocRedRibbon.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Prototype strain of enterovirus, CVB1/Conn-5 ATCC ATCC VR-28 Chemicals, peptides, and recombinant proteins Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat #13778150 Propidium iodide Sigma-Aldrich Cat#P4170; CAS: 25535-16-4 Hoechst 33342 (bisBenzimide H 33342 trihydrochloride) Sigma-Aldrich Cat#14533; CAS: 875756-97-1 Forskolin Sigma-Aldrich Cat#F6886; CAS: 66575-29-9 Beta Krebs Human Cell Design Group Cat#BK-100 SsoAdvanced Universal SYBR Green Supermix Bio-Rad CAT1725274 Critical commercial assays Human Insulin ELISA Mercodia Cat #10-1113-10 Invitrogen TM PureLink TM RNA Micro Scale Kit Invitrogen Cat#12183016 Applied Biosystems TM TaqMan TM Reverse Transcription Reagents Applied Biosystems Cat#4304134 Deposited data eQTL Alonso et al. 17 http://tiger.bsc.es/ pQTL Ferkingstad et al. 21 N/A meQTL McAllan et al. 22 N/A GWAS Chen et al., 11 Suzuki et al., 15 Kurki et al., 23 Yengo et al. 24 N/A Co-localization list, plots and source code This paper https://doi.org/10.5281/zenodo.13987433 Experimental models: Cell lines EndoC-βH1 Ravassard et al. 66 RRID:CVCL_L909 Oligonucleotides AllStars Negative Control siRNA Qiagen Cat #1027281 Human siFUT2, Stealth siRNA Thermofisher Scientific ID:HSS103862 Human SiFUT2#2, Silencer Select siRNA Thermofisher Scientific ID:S533826 Primer for FUT2 Forward: AGCGGCTAGCGAA GATTCA This paper N/A Primer for FUT2 Reverse: GGTCCCAGTGCCT TTGATGT This paper N/A Primer for ACTB Forward: CTGTACGCCAACA CAGTGCT Costa-Junior et al. 67 N/A Primer for ACTB Reverse: GCTCAGGAGGAGC AATGATC Costa-Junior et al. 67 N/A Primer for VAPA Forward: TACCGAAACAAGGA AACTAATGGAA Costa-Junior et al. 67 N/A Primer for VAPA Reverse: GCCTTAAACCTTCA TCTCTCAGGT Costa-Junior et al. 67 N/A Software and algorithms colocRedRibbon This paper https://github.com/antpiron/colocRedRibbon and https://doi.org/10.5281/zenodo.13987433 (version used for this paper) RedRibbon Piron et al. 26 https://github.com/antpiron/RedRibbon (Continued on next page) e1 Cell Genomics 5, 101004, November 12, 2025 OPEN ACCESS ..

    Microarray:

    Article Title: Lung adenocarcinoma surfaceome remodeling with EGFR inhibitors uncovers placental alkaline phosphatase as a target for combination therapy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALPP antibody (8B6) Novus Cat# NB110-3638; RRID:AB_787958) ALPP antibody (EPR6141) Abcam Cat# ab133602; RRID:AB_3065177) β-Actin antibody (8H10D10) Cell Signaling Technology Cat# 3700; RRID:AB_2242334 Phospho-Akt (Ser473) Antibody Cell Signaling Technology Cat# 9271; RRID:AB_329825 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody Cell Signaling Technology Cat# 9101; RRID:AB_331646 Phospho-Stat1 (Tyr701) (58D6) Antibody Cell Signaling Technology Cat# 9167; RRID:AB_561284 Phospho-Stat1 (Ser727) (D3B7) Antibody Cell Signaling Technology Cat# 8826; RRID:AB_2773718 Akt (pan) (C67E7) Antibody Cell Signaling Technology Cat# 4691; RRID:AB_915783 p44/42 MAPK (Erk1/2) (137F5) Antibody Cell Signaling Technology Cat# 4695; RRID:AB_390779 Stat1 (D1K9Y) Antibody Cell Signaling Technology Cat# 14994; RRID:AB_2737027 GAPDH (D16H11) XP Antibody Cell Signaling Technology Cat# 5174; RRID:AB_10622025 FoxO3a (D19A7) Antibody Cell Signaling Technology Cat# 12829; RRID:AB_2636990 Phospho-FoxO3a (Ser294) Antibody Cell Signaling Technology Cat# 5538; RRID:AB_10696878 Phospho-FoxO3a (Ser425) (D5E9T) Antibody Cell Signaling Technology Cat# 64616; RRID:AB_2799662 Biological samples Human Multi-tissue Tissue Microarray (Normal) Novus Biologicals Cat# NBP2-78113 TMA This manuscript The University of Texas MD Anderson Cancer Center Chemicals, peptides, and recombinant proteins FoxO inhibitor MedChemExpress Cat# AS1842856 CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega Cat# G3581 D-Luciferin potassium salt Regis Technologies Inc Cat# 115144-35-9 Gefitinib Cayman Chemical Company Cat# 13166 Lapatinib Cayman Chemical Company Cat# 11493-5 Afatibib Selleck Chemicals Cat# S1011 Osimertinib Selleck Chemicals Cat# S7297 EGFR inhibitor Cayman Chemical Company Cat# 15363 EGFR/ERBB2 inhibitor Cayman Chemical Company Cat# 17568 SsoAdvanced Universal SYBR Green Supermix BIO-RAD Cat# 1725271 16% Formaldehyde, Methanol-free Thermo Fisher Scientific Cat# 28906 DMSO Sigma-Aldrich Cat# D2650 TaqMan Universal Master Mix II, no UNG Applied Biosystems Cat# 4440040 NVP-BKM120 Cayman Chemical Company Cat# 11587 AZD8330 MedChemExpress Cat# HY-12058 SCH772984 MedChemExpress Cat# HY-50846 Critical commercial assays H&E staining kit Abcam Cat# Ab245880 Cell Fractionation kit Cell Signaling Technology Cat# 9038 Pierce Magnetic ChIP kit Thermo Fisher Scientific Cat# 26157 Deposited data PRIDE database This paper PXD069074 (Continued on next page) Cell Reports Medicine 6, 102513, December 16, 2025 e1 ..

    Proliferation Assay:

    Article Title: Lung adenocarcinoma surfaceome remodeling with EGFR inhibitors uncovers placental alkaline phosphatase as a target for combination therapy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALPP antibody (8B6) Novus Cat# NB110-3638; RRID:AB_787958) ALPP antibody (EPR6141) Abcam Cat# ab133602; RRID:AB_3065177) β-Actin antibody (8H10D10) Cell Signaling Technology Cat# 3700; RRID:AB_2242334 Phospho-Akt (Ser473) Antibody Cell Signaling Technology Cat# 9271; RRID:AB_329825 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody Cell Signaling Technology Cat# 9101; RRID:AB_331646 Phospho-Stat1 (Tyr701) (58D6) Antibody Cell Signaling Technology Cat# 9167; RRID:AB_561284 Phospho-Stat1 (Ser727) (D3B7) Antibody Cell Signaling Technology Cat# 8826; RRID:AB_2773718 Akt (pan) (C67E7) Antibody Cell Signaling Technology Cat# 4691; RRID:AB_915783 p44/42 MAPK (Erk1/2) (137F5) Antibody Cell Signaling Technology Cat# 4695; RRID:AB_390779 Stat1 (D1K9Y) Antibody Cell Signaling Technology Cat# 14994; RRID:AB_2737027 GAPDH (D16H11) XP Antibody Cell Signaling Technology Cat# 5174; RRID:AB_10622025 FoxO3a (D19A7) Antibody Cell Signaling Technology Cat# 12829; RRID:AB_2636990 Phospho-FoxO3a (Ser294) Antibody Cell Signaling Technology Cat# 5538; RRID:AB_10696878 Phospho-FoxO3a (Ser425) (D5E9T) Antibody Cell Signaling Technology Cat# 64616; RRID:AB_2799662 Biological samples Human Multi-tissue Tissue Microarray (Normal) Novus Biologicals Cat# NBP2-78113 TMA This manuscript The University of Texas MD Anderson Cancer Center Chemicals, peptides, and recombinant proteins FoxO inhibitor MedChemExpress Cat# AS1842856 CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega Cat# G3581 D-Luciferin potassium salt Regis Technologies Inc Cat# 115144-35-9 Gefitinib Cayman Chemical Company Cat# 13166 Lapatinib Cayman Chemical Company Cat# 11493-5 Afatibib Selleck Chemicals Cat# S1011 Osimertinib Selleck Chemicals Cat# S7297 EGFR inhibitor Cayman Chemical Company Cat# 15363 EGFR/ERBB2 inhibitor Cayman Chemical Company Cat# 17568 SsoAdvanced Universal SYBR Green Supermix BIO-RAD Cat# 1725271 16% Formaldehyde, Methanol-free Thermo Fisher Scientific Cat# 28906 DMSO Sigma-Aldrich Cat# D2650 TaqMan Universal Master Mix II, no UNG Applied Biosystems Cat# 4440040 NVP-BKM120 Cayman Chemical Company Cat# 11587 AZD8330 MedChemExpress Cat# HY-12058 SCH772984 MedChemExpress Cat# HY-50846 Critical commercial assays H&E staining kit Abcam Cat# Ab245880 Cell Fractionation kit Cell Signaling Technology Cat# 9038 Pierce Magnetic ChIP kit Thermo Fisher Scientific Cat# 26157 Deposited data PRIDE database This paper PXD069074 (Continued on next page) Cell Reports Medicine 6, 102513, December 16, 2025 e1 ..

    Staining:

    Article Title: Lung adenocarcinoma surfaceome remodeling with EGFR inhibitors uncovers placental alkaline phosphatase as a target for combination therapy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALPP antibody (8B6) Novus Cat# NB110-3638; RRID:AB_787958) ALPP antibody (EPR6141) Abcam Cat# ab133602; RRID:AB_3065177) β-Actin antibody (8H10D10) Cell Signaling Technology Cat# 3700; RRID:AB_2242334 Phospho-Akt (Ser473) Antibody Cell Signaling Technology Cat# 9271; RRID:AB_329825 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody Cell Signaling Technology Cat# 9101; RRID:AB_331646 Phospho-Stat1 (Tyr701) (58D6) Antibody Cell Signaling Technology Cat# 9167; RRID:AB_561284 Phospho-Stat1 (Ser727) (D3B7) Antibody Cell Signaling Technology Cat# 8826; RRID:AB_2773718 Akt (pan) (C67E7) Antibody Cell Signaling Technology Cat# 4691; RRID:AB_915783 p44/42 MAPK (Erk1/2) (137F5) Antibody Cell Signaling Technology Cat# 4695; RRID:AB_390779 Stat1 (D1K9Y) Antibody Cell Signaling Technology Cat# 14994; RRID:AB_2737027 GAPDH (D16H11) XP Antibody Cell Signaling Technology Cat# 5174; RRID:AB_10622025 FoxO3a (D19A7) Antibody Cell Signaling Technology Cat# 12829; RRID:AB_2636990 Phospho-FoxO3a (Ser294) Antibody Cell Signaling Technology Cat# 5538; RRID:AB_10696878 Phospho-FoxO3a (Ser425) (D5E9T) Antibody Cell Signaling Technology Cat# 64616; RRID:AB_2799662 Biological samples Human Multi-tissue Tissue Microarray (Normal) Novus Biologicals Cat# NBP2-78113 TMA This manuscript The University of Texas MD Anderson Cancer Center Chemicals, peptides, and recombinant proteins FoxO inhibitor MedChemExpress Cat# AS1842856 CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega Cat# G3581 D-Luciferin potassium salt Regis Technologies Inc Cat# 115144-35-9 Gefitinib Cayman Chemical Company Cat# 13166 Lapatinib Cayman Chemical Company Cat# 11493-5 Afatibib Selleck Chemicals Cat# S1011 Osimertinib Selleck Chemicals Cat# S7297 EGFR inhibitor Cayman Chemical Company Cat# 15363 EGFR/ERBB2 inhibitor Cayman Chemical Company Cat# 17568 SsoAdvanced Universal SYBR Green Supermix BIO-RAD Cat# 1725271 16% Formaldehyde, Methanol-free Thermo Fisher Scientific Cat# 28906 DMSO Sigma-Aldrich Cat# D2650 TaqMan Universal Master Mix II, no UNG Applied Biosystems Cat# 4440040 NVP-BKM120 Cayman Chemical Company Cat# 11587 AZD8330 MedChemExpress Cat# HY-12058 SCH772984 MedChemExpress Cat# HY-50846 Critical commercial assays H&E staining kit Abcam Cat# Ab245880 Cell Fractionation kit Cell Signaling Technology Cat# 9038 Pierce Magnetic ChIP kit Thermo Fisher Scientific Cat# 26157 Deposited data PRIDE database This paper PXD069074 (Continued on next page) Cell Reports Medicine 6, 102513, December 16, 2025 e1 ..

    Cell Fractionation:

    Article Title: Lung adenocarcinoma surfaceome remodeling with EGFR inhibitors uncovers placental alkaline phosphatase as a target for combination therapy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALPP antibody (8B6) Novus Cat# NB110-3638; RRID:AB_787958) ALPP antibody (EPR6141) Abcam Cat# ab133602; RRID:AB_3065177) β-Actin antibody (8H10D10) Cell Signaling Technology Cat# 3700; RRID:AB_2242334 Phospho-Akt (Ser473) Antibody Cell Signaling Technology Cat# 9271; RRID:AB_329825 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody Cell Signaling Technology Cat# 9101; RRID:AB_331646 Phospho-Stat1 (Tyr701) (58D6) Antibody Cell Signaling Technology Cat# 9167; RRID:AB_561284 Phospho-Stat1 (Ser727) (D3B7) Antibody Cell Signaling Technology Cat# 8826; RRID:AB_2773718 Akt (pan) (C67E7) Antibody Cell Signaling Technology Cat# 4691; RRID:AB_915783 p44/42 MAPK (Erk1/2) (137F5) Antibody Cell Signaling Technology Cat# 4695; RRID:AB_390779 Stat1 (D1K9Y) Antibody Cell Signaling Technology Cat# 14994; RRID:AB_2737027 GAPDH (D16H11) XP Antibody Cell Signaling Technology Cat# 5174; RRID:AB_10622025 FoxO3a (D19A7) Antibody Cell Signaling Technology Cat# 12829; RRID:AB_2636990 Phospho-FoxO3a (Ser294) Antibody Cell Signaling Technology Cat# 5538; RRID:AB_10696878 Phospho-FoxO3a (Ser425) (D5E9T) Antibody Cell Signaling Technology Cat# 64616; RRID:AB_2799662 Biological samples Human Multi-tissue Tissue Microarray (Normal) Novus Biologicals Cat# NBP2-78113 TMA This manuscript The University of Texas MD Anderson Cancer Center Chemicals, peptides, and recombinant proteins FoxO inhibitor MedChemExpress Cat# AS1842856 CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega Cat# G3581 D-Luciferin potassium salt Regis Technologies Inc Cat# 115144-35-9 Gefitinib Cayman Chemical Company Cat# 13166 Lapatinib Cayman Chemical Company Cat# 11493-5 Afatibib Selleck Chemicals Cat# S1011 Osimertinib Selleck Chemicals Cat# S7297 EGFR inhibitor Cayman Chemical Company Cat# 15363 EGFR/ERBB2 inhibitor Cayman Chemical Company Cat# 17568 SsoAdvanced Universal SYBR Green Supermix BIO-RAD Cat# 1725271 16% Formaldehyde, Methanol-free Thermo Fisher Scientific Cat# 28906 DMSO Sigma-Aldrich Cat# D2650 TaqMan Universal Master Mix II, no UNG Applied Biosystems Cat# 4440040 NVP-BKM120 Cayman Chemical Company Cat# 11587 AZD8330 MedChemExpress Cat# HY-12058 SCH772984 MedChemExpress Cat# HY-50846 Critical commercial assays H&E staining kit Abcam Cat# Ab245880 Cell Fractionation kit Cell Signaling Technology Cat# 9038 Pierce Magnetic ChIP kit Thermo Fisher Scientific Cat# 26157 Deposited data PRIDE database This paper PXD069074 (Continued on next page) Cell Reports Medicine 6, 102513, December 16, 2025 e1 ..

    Chromatin Immunoprecipitation:

    Article Title: Lung adenocarcinoma surfaceome remodeling with EGFR inhibitors uncovers placental alkaline phosphatase as a target for combination therapy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ALPP antibody (8B6) Novus Cat# NB110-3638; RRID:AB_787958) ALPP antibody (EPR6141) Abcam Cat# ab133602; RRID:AB_3065177) β-Actin antibody (8H10D10) Cell Signaling Technology Cat# 3700; RRID:AB_2242334 Phospho-Akt (Ser473) Antibody Cell Signaling Technology Cat# 9271; RRID:AB_329825 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody Cell Signaling Technology Cat# 9101; RRID:AB_331646 Phospho-Stat1 (Tyr701) (58D6) Antibody Cell Signaling Technology Cat# 9167; RRID:AB_561284 Phospho-Stat1 (Ser727) (D3B7) Antibody Cell Signaling Technology Cat# 8826; RRID:AB_2773718 Akt (pan) (C67E7) Antibody Cell Signaling Technology Cat# 4691; RRID:AB_915783 p44/42 MAPK (Erk1/2) (137F5) Antibody Cell Signaling Technology Cat# 4695; RRID:AB_390779 Stat1 (D1K9Y) Antibody Cell Signaling Technology Cat# 14994; RRID:AB_2737027 GAPDH (D16H11) XP Antibody Cell Signaling Technology Cat# 5174; RRID:AB_10622025 FoxO3a (D19A7) Antibody Cell Signaling Technology Cat# 12829; RRID:AB_2636990 Phospho-FoxO3a (Ser294) Antibody Cell Signaling Technology Cat# 5538; RRID:AB_10696878 Phospho-FoxO3a (Ser425) (D5E9T) Antibody Cell Signaling Technology Cat# 64616; RRID:AB_2799662 Biological samples Human Multi-tissue Tissue Microarray (Normal) Novus Biologicals Cat# NBP2-78113 TMA This manuscript The University of Texas MD Anderson Cancer Center Chemicals, peptides, and recombinant proteins FoxO inhibitor MedChemExpress Cat# AS1842856 CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega Cat# G3581 D-Luciferin potassium salt Regis Technologies Inc Cat# 115144-35-9 Gefitinib Cayman Chemical Company Cat# 13166 Lapatinib Cayman Chemical Company Cat# 11493-5 Afatibib Selleck Chemicals Cat# S1011 Osimertinib Selleck Chemicals Cat# S7297 EGFR inhibitor Cayman Chemical Company Cat# 15363 EGFR/ERBB2 inhibitor Cayman Chemical Company Cat# 17568 SsoAdvanced Universal SYBR Green Supermix BIO-RAD Cat# 1725271 16% Formaldehyde, Methanol-free Thermo Fisher Scientific Cat# 28906 DMSO Sigma-Aldrich Cat# D2650 TaqMan Universal Master Mix II, no UNG Applied Biosystems Cat# 4440040 NVP-BKM120 Cayman Chemical Company Cat# 11587 AZD8330 MedChemExpress Cat# HY-12058 SCH772984 MedChemExpress Cat# HY-50846 Critical commercial assays H&E staining kit Abcam Cat# Ab245880 Cell Fractionation kit Cell Signaling Technology Cat# 9038 Pierce Magnetic ChIP kit Thermo Fisher Scientific Cat# 26157 Deposited data PRIDE database This paper PXD069074 (Continued on next page) Cell Reports Medicine 6, 102513, December 16, 2025 e1 ..



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